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Image Search Results
Journal: Aging (Albany NY)
Article Title: Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
doi: 10.18632/aging.202895
Figure Lengend Snippet: MiRNA-150 promotes tumor growth and activates Wnt and Hh signaling in vivo . ( A ) Tumor growth curves of volumes measured twice weekly after the injection of miRNA-150 inhibitors. Images of xenograft tumors and nude mice (N=6) and H&E stainings are shown. ( B ) Tumor weights after resection from nude mice. ( C , D ) The expression levels of miRNA-150 and Hh and Wnt downstream genes were reduced in vivo in tumors treated with miRNA-150 inhibitors. The expression of SUFU was increased in tumors treated with miRNA-150 inhibitors (NSC=6 tumors, 150-inh=3 tumors). Student’s t -test was performed. *p<0.05.
Article Snippet: The primary antibodies were as followed: Wnt/β-Catenin Activated Targets Antibody Sampler Kit (#8655), EMT Antibody Sampler Kit (#9782),
Techniques: In Vivo, Injection, Expressing
Journal: Aging (Albany NY)
Article Title: Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
doi: 10.18632/aging.202895
Figure Lengend Snippet: MiRNA-150 induces Hh and Wnt signaling activation and EMT via SUFU. ( A ) MiRNA-150 inhibition decreased β-catenin-dependent transcriptional activity, and this decrease was reversed by SUFU siRNAs. A TOPFlash/FOPFlash assay was conducted in BGC-823 cells transfected with miRNA-150 inhibitors or co-transfected with 150-inh and SUFU siRNAs. ( B , C ) Interference of SUFU alleviated the β-catenin cytoplasmic translocation induced by miRNA-150 inhibition. ( B ) β-catenin expression was detected in the cytoplasm and nucleus. ( C ) Confocal immunostaining of β-catenin was performed to visualize the cytoplasmic or nuclear localization of β-catenin. ( D , E ) MiRNA-150 inhibitors reduced the expression of Hh signaling genes and EMT markers, and SUFU siRNA rescued this inhibitory effect. The expression of Hh signaling and EMT proteins was detected in BGC-823 cells by western blotting after transfection with miRNA-150 inhibitors or co-transfection with 150-inh and SUFU siRNAs. ( F ) The mRNA levels of some Hh-, Wnt- and EMT-related genes were reduced under miRNA-150 inhibition, but increased somewhat after SUFU gene knockdown. *p<.05.
Article Snippet: The primary antibodies were as followed: Wnt/β-Catenin Activated Targets Antibody Sampler Kit (#8655), EMT Antibody Sampler Kit (#9782),
Techniques: Activation Assay, Inhibition, Activity Assay, Transfection, Translocation Assay, Expressing, Immunostaining, Western Blot, Cotransfection, Knockdown
Journal: Aging (Albany NY)
Article Title: Dual activation of Hedgehog and Wnt/β-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer
doi: 10.18632/aging.202895
Figure Lengend Snippet: Summary of the findings. Dual activation of the Hh and Wnt/β-catenin signaling pathways is induced by miRNA-150 via the downregulation of SUFU expression in human GC.
Article Snippet: The primary antibodies were as followed: Wnt/β-Catenin Activated Targets Antibody Sampler Kit (#8655), EMT Antibody Sampler Kit (#9782),
Techniques: Activation Assay, Protein-Protein interactions, Expressing